Product profile:
Mycoplasma Detector Kit Is an assay kit specifically for mycoplasma contamination in cell culture that detects at least 125 mycoplasma species using fluorescent PCR probes. There included 104 Mycoplasmas, including Mycoplasma fermentation (M. fermentans), Mycoplasma pig (M. hyorhinis), Mycoplasma oralis (M. oral), Mycoplasma arginine (M. arginini), Mycoplasma verticum. (M. gallisepticum), M. pneumoniae (M. pneumonia), and Mycoplasma synovium (M. synoviae); 5 Acholeplasmas without cholesterticogen (A. laidlawii); Ureaplasma (Ureaplasma urealyticum), 3 Ureaplasmas and 13 Spiroplasmas, There was no cross-reaction with common bacteria in the environment such as Enterobacter, Enterococcus, Staphylococcus, Serratia, Salmonella, Lactobacillus, Candida, Aspergillus sp. The kit is divided into two operation methods: direct expansion method and extraction method. The direct expansion method is simple to use, without mycoplasma DNA extraction, 1 μ L of cell culture medium and 1 μ L of enzyme solution can be directly added to the reaction system for computer detection. The entire experiment was completed in an hour and a half hours. Extraction method can detect the presence of mycoplasma, high sensitivity, for common mycoplasma species, such as M. fermentalis (M. fermentans), M. S. pig (M. hyorhinis), Mycoplasma (M. oral), Mycoplasma arginine (M. arginini), Mycoplasma agalactiae (M. agalactiae), M. bovis) (M. pulmonis). The concentration of 5 CFU / mL. This product is suitable for a variety of suspended, adherent cultured cells, including CHO, Vero, NS0, SP 2 / 0, Sf 9,293, HeLa, and has a wide range of medium compatibility. It can provide simple, rapid and reliable mycoplasma detection experiments for biopharmaceutical enterprises and scientific research institutions.