This kit is specially developed for the Illumina high-throughput sequencing platform. It  provides the enzyme premix system and reaction buffer required for genomic DNA  library construction, including all components except PCR primers. Compared with  traditional library construction kits, this kit uses a novel transposase method for library  construction, which can complete DNA fragmentation, end repair and adapter ligation  in one simple enzymatic reaction, significantly reducing the amount of template used. ,  reducing the experimental operation steps and shortening the library construction time;  using high-fidelity DNA polymerase for library enrichment, unbiased PCR  amplification, expanding the coverage area of the sequence, and efficiently preparing  DNA for the Illumina next-generation sequencing platform library. This kit is suitable for  starting template DNA input of 50 ng. All reagents in the kit have undergone strict  quality control and functional verification to ensure the stability and reproducibility of  library construction to the greatest extent.


Reagents to Be Supplied by User
1.Magnetic stand: DynaMagTM-2 (Cat.No. 12321D) is recommended.
2.DNA purification and recovery kit: It is recommended to use the Kangwei magnetic  bead method DNA purification and recovery kit (Cat.No.CW2508).
3.Library PCR primer kit and PCR thermal cycler: It is recommended to use Kangwei  Transposase Next Generation Sequencing Multi-sample Primer Kit  (Cat.No.CW2958/CW2959/CW2960/CW2961/CW2962/CW2963).
4.Absolute ethanol, deionized water (pH between 7.0-8.0).
7.Reaction tubes: It is recommended to use low adsorption PCR tubes and 1.5 ml  centrifuge tubes.
6.Pipette tips: It is recommended to use high-quality filter tips to prevent  contamination of kits and library samples.