Product Introduction:  
This kit provides a simple, fast and efficient nucleic acid  purification method. The product can be used for the selective or  non-selective recovery of DNA during the construction of NGS  library, as well as the purification and recovery of PCR products. After the CMPure beads are mixed with the sample in a certain  proportion, the magnetic beads selectively adsorb the nucleic  acid. After two steps of washing, the eluted DNA is of high purity.  The A260/A280 ratio is between 1.7-1.9, and the A260/A230 ratio is  usually above 2.0. The DNA purified by this kit is suitable for  PCR, Real-Time PCR, sequencing, southern blotting and other  experiments                 

Preparation before the experiment and important notes:  
1. Freezing, centrifugation, and ultrasound can cause irreversible  damage to the CMPure beads.
2. Magnetic beads in CMPure will aggregate into clusters after  being placed for a long time, so that the surface area of  magnetic beads will be reduced, and the yield of sample  recovery will be reduced. Before use, magnetic beads must be  thoroughly mixed by vortexing.
3. Before use, it is recommended that the CMPure beads should  be vortexed and aliquoted into 1.5 ml microcentrifuge tubes,  each tube containing 1 ml of CMPure beads.
4. This kit is not suitable for the purification of DNA fragments  smaller than 100 bp. If the DNA fragments are smaller than 100  bp, it is recommended to increase the amount of CMPure to 4  times of the sample volume. 5. For the selective recovery of DNA, CMPure is more sensitive to  the concentration of ions in the DNA solution. Because the  3concentration of ions in the adaptered DNA solution and the  PCR product obtained by the NGS library construction kits from  different manufacturers are different, the amount of reagents  used varies.     

Protocol:  
1. Vortex CMPure for 20 seconds to thoroughly mix it into a  homogeneous solution.  
2. Add the purified DNA solution to a 1.5 ml centrifuge tube.
3. Add two times of the sample volume of CMPure to the  centrifuge tube in the previous step. Vortex for 5 seconds and  allow to stand at room temperature for 5 minutes.
4. Place the centrifuge tube from the previous step on the  magnetic stand until the beads are fully adsorbed  (approximately 5 minutes).
5. Keep the centrifuge tube on the magnetic stand and discard  the solution completely, avoiding touch magnetic beads.
6. Continue to hold the tube on the magnetic stand and add 250  μl of freshly prepared 80% ethanol to the tube.
7. Keep the centrifuge tube on the magnetic stand. discard the  ethanol completely after the suspended magnetic beads are  fully absorbed.
8. Repeat step 6-7 twice.  
9. Keep the centrifuge tube on the magnetic stand for 10 minutes  to completely evaporate the ethanol.
10. Remove the centrifuge tube from the magnetic stand and  add 20-100 μl of EB (self-prepared) or ddH2O. After vortexing to completely resuspend the magnetic beads, leave at room  temperature for 5 minutes.
11. Place the centrifuge tube from the previous step on the  magnetic stand until the beads are fully adsorbed  (approximately 5 minutes).
12. Transfer the DNA elution to a new 1.5 ml centrifuge tube. At  this point, magnetic beads can be discarded.